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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
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ISSN : |
1948-5948 |
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DOI : |
10.4172/1948-5948.1000155 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
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Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
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022 $a1948-5948
024 7 $a10.4172/1948-5948.1000155$2DOI
100 1 $aALEM, D.
245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource]
260 $c2014
520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.
650 $aACTIVIDAD ANTIMICROBIANA
650 $aALTERNARIA
650 $aBIOTECNOLOGÍA
650 $aPÉPTIDOS
700 1 $aDIAZ, P.
700 1 $aLEONI, C.
700 1 $aDE SIMONE, S.G.
700 1 $aCORREA, A.
700 1 $aOPPEZZO, P.
700 1 $aDALLA RIZZA, M.
773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 18 | |
| 2. |  | Tomaso, J.C.; Díaz P., A. Influencia de las fechas de siembra sobre los rendimientos y algunos parámetros de calidad de malta ln: Congreso de cebada maltera, 2., 1996, Carillanca, Temuco, Chile Beratto M., E., ed. Trabajos presentados. Temuco: FAO/INIA Carillanca, 1999. p. 175-192| Biblioteca(s): INIA La Estanzuela. |
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| 3. | | ALONSO, M.; DÍAZ, P.; CARBALLO, S.; PAGANI, C. Contaminación microbiana en lechugas y tomates ln: Congreso Nacional de Horticultura, 8.; Seminario Regional de Frutilla, 2001, Salto, Uruguay. Resúmenes. Montevideo (Uruguay): SUH; INIA, 2001. p. 40 Sociedad Uruguaya de Horticultura; Instituto Nacional de Investigación Agropecuaria, Uruguay.| Biblioteca(s): INIA Las Brujas; INIA Salto Grande; INIA Tacuarembó; INIA Treinta y Tres. |
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| 9. | | MANZI, M.; BORSANI, O.; DÍAZ, P.; RIVAS, F. Relationship between Flower Intensity, Oxidative Damage and Protection in Citrus under Water Stress Conditions. Acta Horticulturae, 2015, no.1065, p. 1243-1250. [Conference Paper] Proc. XII International Citrus Congress - International Society of Citriculture. Eds. B. Sabater-Muñoz, P. Moreno, L. Peña, L. Navarro (3 vols.)| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 10. | | DIAZ, P.; CABRERA, A.; ALEM, D.; LARRAÑAGA, P.; FERREIRA, F.; DALLA RIZZA, M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. [Actividad antifúngica de extractos de plantas medicinales contra el hongo fitopatógeno alternaria spp.] Chilean Journal of Agricultural Research, 2011, v.71, no.2, p.231-239. doi: http://dx.doi.org/10.4067/S0718-58392011000200008 2-s2.0-79960343579 RESEARCH. Received: 2 August 2010. Accepted: 26 December 2010.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 3 |
| Biblioteca(s): INIA Las Brujas. |
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| 11. | | DALLA RIZZA, M.; DIAZ, P.; NARANCIO, R.; CABRERA, A.; FERREIRA, F. Biomolecules as host defense weapons against microbial pathogens. (Review) Recent Patents on DNA and Gene Sequences, 2008, v.2, no.2, p.82-96. Article history: Received: April 21, 2008 / Accepted: April 29, 2008 / Revised: April 29, 2008.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - - |
| Biblioteca(s): INIA Las Brujas. |
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| 12. | | BORSANI, O.; CASARETTO, E.; MÁRQUEZ, A.J.; REBUFFO, M.; DÍAZ, P.; MONZA, J. Metabolismo de prolina en cultivares de Trifolium pratense con diferente producción estival. In: BONETE, M.J.; MARTÍNEZ-ESPINOSA, R.M. (Coord.). Avances en el metabolismo del nitrógeno: de la genómica y la proteómica a las aplicaciones agronómicas, industriales y medioambientales. [San Vicente, Alicante], ES: Club Universitario, 2010. p. 319-325.| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela. |
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| 13. | | ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. Article history: Received September 7 2010. /Accepted June 21, 2011.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 3 |
| Biblioteca(s): INIA La Estanzuela. |
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| 16. | | LARRAÑAGA, P.; DIAZ, P.; CABRERA, A.; ALEM, D.; ALMEIDA SOUZA, A.L.; GIOVANNI DE SIMONE, S.; DALLA RIZZA, M. Activity of naturally derived antimicrobial peptides against filamentous fungi relevant for agriculture . Sustainable Agriculture Research, 2012, v. 1, no.2, p. 211-221. Article history: Received: June 12, 2012 / Accepted: July 10, 2012 / Online Published: July 23, 2012.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : C - 0 |
| Biblioteca(s): INIA Las Brujas. |
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| 17. | | ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 4 |
| Biblioteca(s): INIA Las Brujas. |
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| 18. | | REBUFFO, M.; ZARZA, R.; BORSANI, O.; CASARETTO, E.; MESSA, A.; SAINZ, M.M.; SALDIAS, R.; ALZUGARAY, R.; CONDON, F.; DIAZ, P.; MONZA, J.; RISSO, D.; BEMHAJA, M.; BERMÚDEZ, R.; AYALA, W.; ALTIER, N.; ZARZA, M. Genetic resources of forage legumes for agriculture-pastoral system in Uruguay. Lotus Newsletter, 2007, Volume 37, Issue 2, pages 56.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 18 | |
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